Protein crystallography, Structural Biology, Biochemistry.
Our lab is interested in determining the three-dimensional structure of macromolecules by X-ray Crystallography. Also we employ other biophysical and biochemical techniques to understand the molecular mechanism of important proteins. Proteins of our interest are: • Proteins that are essential for survival and growth of bacteria, yeast etc. Three dimensional structures of these proteins will be determined by X-ray crystallography and subsequently used as a model for designing drugs, specific to the target as well as for the pathogenesis. • Molecular characterization, structure-function relationship of enzymes that is useful for industrial purpose • Identification and characterization of proteins with a novel fold.
- Kaur, A., Gautam, R., Srivastava, R., Chandel, R., Kumar, A, Karthikeyan, S.*, and Bachhawat, A. K.* (2017). ChaC2: An enzyme for Slow Turnover of Cytosolic Glutathione. J. Biol. Chem. 292, 638-651.
- Yadav, S. and Karthikeyan, S.* (2015). Structural and biochemical characterization of GTP cyclohydrolase II from Helicobacter pylori reveals its redox dependent catalytic activity. J. Struct. Biol. 192, 100-115.
- Islam, Z., Kumar, A., Singh, S., Salmon. L., and Karthikeyan, S.* (2015). Structural Basis for Competitive Inhibition of 3,4-Dihydroxy-2-butanone-4-phosphate Synthase from Vibrio cholera. J. Biol. Chem. 290, 11293-11308.
- Singh, M., Kumar, P., Yadav, S., Gautam, R., Sharma, N., and Karthikeyan, S.* (2013). The crystal structure reveals the molecular mechanism of bifunctional 3,4-dihydroxy-2-butanone 4-phosphate synthase/GTP cyclohydrolase II (Rv1415) from Mycobacterium tuberculosis Acta Cryst. D69, 1633-1644.
- MKumar, P., Singh, M., and Karthikeyan, S.* (2011). Crystal structure analysis of icosahedral lumazine synthase from Salmonella typhimurium, an antibacterial drug target. Acta Cryst. D67, 131-139.