PhD (1991-1997): Dr. Ranajit K Ghosh, Indian Institute of Chemical Biology, CSIR
Post doctoral experiences (1997-2000):
1) Late Prof. Edward James (Jim) Ofengand (1997-1999): Biogenesis of Pseudouridine
2) Dr. Richard (Rich) L Hurwitz (1999-2000): Ocular Gene Therapy, Retinoblastoma, Stargardt’s disease
Biogenesis of Pseudouridine and Ribosomology: An Escherichia coli perspective
The project was primarily focused on the functional characterization of different family of pseudouridine synthases in Escherichia coli.
Ref no: 2, 3 and 4
Ocular Gene therapy, Adenoviral transduction and involvement of CD44
This project is translational in nature, where gene therapy is taken into account of ameliorating progression of retinoblastoma. We have also tried to correct ocular genetic disorders by providing the correct gene through lenti-viral gene delivery system.
Ref no: 9
Biology of Vibrio cholerae and related Vibrios: emergence of multidrug resistant strains, quorum sensing regulatory small RNAs, master regulators and natural variants, metabolic fitness
One of our main goals is to understand quorum sensing a “cell to cell” communication process by employing Vibrio cholerae as a model organism; gain further molecular insight into various components of such intricate cellular networking process by analyzing natural variants. In case of metabolic fitness, we have discovered the existence of ethanolamine utilization machinery in Vibrio species. Presently, we are investigating the significance of such metabolic pathway in the survival and virulence of such Vibrio strains.
Ref no: 5, 6, 7, 8, 10, 13, 14, 15, 18, 19 and 20
Type III effector molecules, WH2 domain proteins and model systems: Gaining functional insight of bacterial proteins exploiting model organisms
In this project, our aim is to develop yeast as a model system to study bacterial effector proteins deliver through T3SS.
Ref no: 12, 17 and 22
Bacterial ecology, cross domain cross talk:
Ref no: 8 and 11
Biology of Commensal E. coli and other intestinal pathogens
Ref no: 16, 21
Publications year wise:
1. Pajni S, Sharma C, Basing N, Ghosh A, Ramamurthy T, Nair GB, Ramajayam S, Das B, Kar S, Roychowdhury S, et al. Studies on the genesis of Vibrio cholerae O139: Identification of probable progenitor strains. J Med Microbiol. 1995 Jan; 42(1): 20-5.
2. Raychaudhuri S, Conrad J, Hall BG, Ofengand J. A pseudouridine synthase required for the formation of two universally conserved pseudouridines in ribosomal RNA is essential for normal growth of Escherichia coli. RNA. 1998 Nov; 4(11): 1407-17.
3. Raychaudhuri S, Niu L, Conrad J, Lane BG, Ofengand J. Functional effect of deletion and mutation of the Escherichia coli ribosomal RNA and tRNA pseudouridine synthase RluA. J Biol Chem. 1999 Jul; 274(27): 18880-6.
4. Gutgsell NS, Del Campo MD, Raychaudhuri S, Ofengand J. A second function for pseudouridine synthases: A point mutant of RluD unable to form pseudouridines 1911, 1915, and 1917 in Escherichia coli 23S ribosomal RNA restores normal growth to an RluD-minus strain. RNA. 2001 Jul; 7(7): 990-8.
5. M.Thungapathra, Amita, Kislay K.Sinha, Saumya Ray Chaudhuri, Pallavi Garg, T.Ramamurthy, G.B.Nair and Amit Ghosh: Occurrence of Antiobiotic Resistance Gene Cassettes aac (6')-Ib, dfrA5, dfrA12, and ere A2 in Class I Integrons in Non 01, Non-0139 Vibrio cholerae Strains in India. Antimicrobial Agents and Chemotherapy, 2002, Sept; 46(9): 2948-2955.
6. Amita, S Roy Chowdhury, M.Thungapathra, T. Ramamurthy, G.Balakrish Nair and Amit Ghosh: Class I Integrons and SXT elements in El Tor strains Isolated before and after 1992 Vibrio cholerae 0139 Outbreak, Calcutta, India. Emerging Infectious Diseases. 2003 April: 9 (4): 500-502.
7. Saumya Raychaudhuri, Vibhu Jain and Mitesh Dongre: Identification of a constitutively active variant of LuxO that affects production of HA/protease and biofilm development in a non-O1, non- O139 Vibrio cholerae O110. Gene, 2006 Mar 15; 369:126-33.
8. Vibhu Jain, Mitesh Dongre and Saumya Raychaudhuri: Interaction of Vibrio cholerae O139 with an intestinal parasite Entameoba histolytica. J Med Microbiol, 2006 Dec ; 55: 1755-6.
9. Saumya Ray Chaudhuri, Joshua N Mallam, Patricia Chévez-Barrios, Lalita Wadhwa1,Philip Ng, Mary Y. Hurwitz, and Richard L. Hurwitz: Modulation of Adenoviral Transduction In Vitro and In Vivo by Hyaluronan and its receptor CD44. Molecular Therapy 2007 Mar;15(3):566-70.
10. Mitesh Dongre, Ranjana Tripathy, Vibhu Jain and Saumya Raychaudhuri: Functional independence of a variant LuxOPL91 from a non-O1, non-O139 V. cholerae over the activity of CsrA and Fis. J Med Microbiol 2008, 57: 1041-1045.
11. Hadi Abd, Soni Priya Valeru, Susan Marouf Sami, Amir Saeed, Saumya Raychaudhuri, Gunnar Sandström. Interaction between Vibrio mimicus and Acanthamoeba castellanii. Environ Microbiol Rep. 2010 2(1):166-171.
12. Ranjana Tripathi, Chetna Dureja, Swati Haldar, Alok K Mondal, and Saumya Raychaudhuri. VopF, a type III effector protein from a non-O1, non-O139 V. cholerae strain demonstrates toxicity in Saccharomyces cerevisiae model. J Med Microbiol 2010 ; 59(Pt 1):17-24.
13. Dongre M, Khatri N, Dureja C, Raychaudhuri S. Alanine-scanning mutagenesis of selected residues in the N-terminal region alters the functionality of LuxO: lessons from a natural variant LuxOPL91. J Med Microbiol. 2011 Jun; 60(Pt 6):856-60.
14. Dongre M, Singh NS, Dureja C, Pedda N, Solanki AK, Ashish , Raychaudhuri S. Evidence on how a conserved glycine in the hinge region of HapR regulates its DNA binding ability: lessons from a natural variant. J Biol Chem. 2011 Apr 29; 286 (17):15043-9.
15. Khatri N, Khatri I, Subramanian S, Raychaudhuri S. Ethanolamine utilization in Vibrio alginolyticus. Biol Direct. 2012 Dec 12; 7 (1):45. [Epub ahead of print]
16. Indu Khatri, Chetna Dureja, Saumya Raychaudhuri, and Srikrishna Subramanian. Draft Genome Sequence of the Opportunistic Human Pathogen Morganella morganii SC01. Genome Announc. January 2013 1:e00051-12; doi:10.1128/genomeA.00051-12 http://genomea.asm.org/content/1/1/e00051-12.abstract.html?etoc
17. Tripathi R, Kaithwas V, Dureja C, Raychaudhuri S. Alanine-scanning mutagenesis of WH2 domains of VopF reveals residues important for conferring lethality in a Saccharomyces cerevisiae model. Gene. 2013 Aug 1; 525(1):116-23. doi: 10.1016/j.gene.2013.04.071. Epub 2013 May 13.
18. Khatri I, Mahajan S, Dureja C, Subramanian S, Raychaudhuri S. Evidence of a new metabolic capacity in an emerging diarrheal pathogen: lessons from the draft genomes of Vibrio fluvialis strains PG41 and I21563. Gut Pathog. 2013 Jul 29; 5 (1):20. [Epub ahead of print]
19. Singh R, Rathore YS, Singh NS, Peddada N, Ashish, Raychaudhuri S. Substitution of Glutamate Residue by Lysine in the Dimerization Domain Affects DNA Binding Ability of HapR by Inducing Structural Deformity in the DNA Binding Domain. PLoS One. 2013 Oct 14;8(10):e76033. doi: 10.1371/journal.pone.0076033.
20. Naorem Santa Singh, Sangita Kachhap, Richa Singh, Rahul Chandra Mishra, Balvinder Singh , Saumya Raychaudhuri.The length of glycine rich linker in DNA binding domain is critical for optimal functioning of quorum sensing master regulatory protein HapR. Mol Genet Genomics. 2014 Dec;289(6):1171-82. doi: 10.1007/s00438-014-0878-5. Epub 2014 Jul 5.
21. Dureja C, Mahajan S, Raychaudhuri S. Phylogenetic Distribution and Prevalence of Genes Encoding Class I Integrons and CTX-M-15 Extended-Spectrum β-Lactamases in Escherichia coli Isolates from Healthy Humans in Chandigarh, India. PLoS One. 2014 Nov 19;9(11):e112551. doi: 10.1371/journal.pone.0112551. eCollection 2014.
22. Bankapalli LK, Mishra RC, Singh B, Raychaudhuri S. Identification of Critical Amino Acids Conferring Lethality in VopK, a Type III Effector Protein of Vibrio cholerae: Lessons from Yeast Model System. PLoS One. 2015 Oct 21; 10(10):e0141038. doi: 10.1371/journal.pone.0141038. eCollection 2015.
23. Gahlawat G, Shikha S, Chaddha BS, Chaudhuri SR, Mayilraj S, Choudhury AR. Microbial glycolipoprotein-capped silver nanoparticles as emerging antibacterial agents against cholera. Microb Cell Fact. 2016 Feb 1;15(1):25. doi: 10.1186/s12934-016-0422-x.