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Name:
Dr. Pawan Gupta
Expertise:
Molecular Immunology, Cell Biology, Infectious Disease, Proteomics, Protein Science & Engineering

Contact Address:

Institute of Microbial Technology,
Sector 39-A, Chandigarh 160036, INDIA
Phone: +91-9317703783; +91-172-6665221;
Fax: +91-172-2690585.
Email: pawan@imtech.res.in

Write-up of research and development interests/focus, past and present goals:

Host-Pathogen Interaction (Mycobacterium tuberculosis, Mtb):
Mtb is the etiologic agent of tuberculosis in humans and is responsible for more morbidity than any other bacterial disease. We are investigating host-pathogen interaction, in context of host lipid sensing nuclear receptor and pathogen lipids, contributing towards Mtb pathogenesis or clearance. We are also investigating transcription factors modulation of macrophage polarization, plasticity and their role as as combats or cohorts to Mtb survival. We plan to make an expression atlas of nuclear receptor in CD4+, CD8+. CD14+, CD19+ immune cells derived from pulmonary tuberculosis patients, close contacts and healthy individuals.
Autoimmune Disorders and Immune Cells Plasticity
We are using autoimmune mice models of rheumatoid arthritis (RA), allergic asthma (AA) and encephalomyelitis (EAE) to study differential expression and function of nuclear receptor in plasticity, polarization and activation of T cell, B cell, DCs and macrophage.
Neuron and Cancer biology
We plan to investigate molecular circuitry of neuron function, differentiation and cancer at the level of nuclear receptor and associated coactivators and corepressors.
Post-Translational Modification (PTM)
We are deciphering PTM to, and complex regulation of, orphan/nuclear receptors. Also proteome-vide screening for Retinoic acid, Vitamin B6 and Acrolein conjugation as novel PTM.
SMART and Switchable Therapeutic Proteins
We are working on Selective Mutagenesis and Rational Transplantation (SMART) of therapeutic proteins. We are attempting oral/bronchial protein vehicle from bromelain. Next generation switchable recombinant erythropoietin, insulin, growth factors and cytokines. Protein Nucleic Acid (PNA) Mimetics for selective anti-gene/-sense therapy towards Mtb is also being attempted.


Significant recognition: Awards, fellowships, international funding of distinction, technologies transferred/licensed etc.:

  • 1st Prize in the theme of Indigenous Science and Technology in the Young Scientist Conference of India International Science Festival (IISF 2015), held at IIT Delhi in Dec 2015.
  • 4th BD- TCS Award 2012
  • DBT-Innovative Young Biotechnologist Award 2009
  • Awarded DST Travel Award in 2009
  • Awarded ICMR Travel Award in 2010
  • Awarded US Department of State BEP Travel Award in 2010

Selected list of Publications and Patents:

2015-2016 

  • Saini, A., Mahajan, S., Gupta, P*. (2016) Nuclear Receptor Expression Atlas in BMDCs: Nr4a2 restricts immunogenicity of BMDCs and impedes EAE. European Journal of Immunology. 46(8), 1842-53 Impact Factor: 4.18. Highlighted as In This Issue
  • Mahajan, S., Saini, A., Kalra, R. and Gupta, P.* (2016) Frienemies of infection: A chronic case of host nuclear receptors acting as cohorts or combatants of infection. Crit Rev Microbiol, 42(4), 526-34.  Impact Factor: 8.19.
  • Bhagyaraj, E., Nanduri, R., Saini, A., Dkhar, H.K., Ahuja, N., Chandra, V., Mahajan, S., Kalra, R., Tiwari, D., Sharma, C., Janmeja, A.K., Gupta, P*. (2016) Human Xenobiotic Nuclear Receptor PXR Augments Mycobacterium tuberculosis Survival. Journal of Immunology. 197(1), 244-55Impact Factor: 4.98.
  • Chandra, V., Bhagyaraj, E., Parkesh, R. and Gupta, P*. (2016) Transcription factors and cognate signalling cascades in the regulation of autophagy. Biol Rev Camb Philos Soc. May 91(2), 429-51. Impact Factor: 10.725.
  • Sethi, D., Mahajan, S., Singh, C., Lama, A., Hade, M.D., Gupta, P., Diskshit, K.L. (2016) Lipoprotein, LprI, of Mycobacterium tuberculosis acts as a lysozyme inhibitor. J Biol Chem. 291, 2938-53. Impact Factor: 4.26.
  • Kaur, S., Rana, V., Singh, P., Trivedi, G., Anand, S., Kaur, A., Gupta, P., Sharma, C., (2016) Novel Mutations Conferring Resistance to Kanamycin in Mycobacterium tuberculosis clinical isolates from Northern India. Tuberculosis. 96, 96-101. Impact Factor: 2.95. 
  • Nanduri, R., Bhutani, I., Somavarapu, A.K., Mahajan, S., Parkesh, R.*, Gupta, P*. (2015) ONRLDB-manually curated database of experimentally validated ligands for orphan nuclear receptors. Database (Oxford). Dec 4;2015. pii: bav112. doi: 10.1093/database/bav112. Impact Factor: 3.37.
  • Chandra, V., Bhagyaraj, E., Nanduri, R., Ahuja, N., Gupta, P*. (2015) NR1D1 ameliorates Mycobacterium tuberculosis clearance through regulation of autophagy. Autophagy. 11, 1987-97. Impact Factor: 11.75.
  • Mahajan,S., Saini, A., Chandra, V., Nanduri, R., Kalra, R., Bhagyaraj, E., Khatri, N. and Gupta, P*. (2015) Nuclear receptor Nr4a2 promotes alternative polarization of macrophages and confer protection in sepis. J Biol Chem. 290, 18304-14. Impact Factor: 4.57. Best of the Year JBC 2015.
  • Hoque, M., Nanduri, R., Gupta, J., Mahajan, S., Gupta, P. and Saleemuddin, M. (2015) Oleic acid complex of bovine α-lactalbumin induces eryptosis in human and other erythrocytes by a ca2+-independent mechanism. Biochim Biophys Acta-General Subjects. 1850, 1729-39. Impact Factor: 3.83.
  • Nanduri, R., Mahajan, S., Bhagyaraj, E., Sethi, K., Kalra, R., Chandra, V. and Gupta, P*. (2015) The active form of vitamin D transcriptionally represses the Smad7 signaling and activates ERK to inhibit the differentiation of a inflammatory T helper cell subset and suppress experimental autoimmune encephalomyelitis. J Biol Chem. 290, 12222-36. Impact Factor: 4.57.
  • Loharch, S., Bhutani, I., Jain, K., Gupta, P., Sahoo, D.K. and Parkesh, R. (2015) EpiDBase: a manually curated database for small molecule modulators of epigenetic landscape. Database (Oxford). Mar 16. pii: bav013. doi: 10.1093/database/bav013. Impact Factor: 3.37.
  • Dkhar, H.K., Gopalsamy, A., Loharch, S., Kaur, A., Bhutani, I., Saminathan, K., Bhagyaraj, E., Chandra, V., Swaminathan, K., Agrawal, P., Parkesh, R.* and Gupta P*. (2015) Discovery of Mycobacterium tuberculosis alpha-1,4-glucan branching enzyme (GlgB) inhibitors by structure- and ligand-based virtual screening. J Biol Chem, 290, 76-89. Impact Factor: 4.57.
  • Bhutani, I., Loharch, S., Gupta, P., Madathil, R. and Parkesh, R. (2015) Structure, Dynamics, and Interaction of Mycobacterium tuberculosis (Mtb) DprE1 and DprE2 Examined by Molecular Modeling, Simulation, and Electrostatic Studies. PLoS One, 10, e0119771. Impact Factor: 3.53.
  • Batra, M., Sharma, R., Chandra, V., Aggarwal, M., Agarwal, U., Gupta, P., Singh, R.P. and Tomar, S. (2015) In silico and proteomic analysis of protein methyltransferase CheR from Bacillus subtilis. Int J Biol Macromol. 77, 168-180. Impact Factor: 3.1.

Full list of publications on Google Scholar and Research Gate

 

https://scholar.google.com/citations?user=zU1Y8-MAAAAJ&hl=en 

 

https://www.researchgate.net/profile/Pawan_Gupta11/contributions 



Present group members:

Ph. D students:

Ravikanth Nanduri (Ph.D.), Ella Bhagyaraj, Rashi Kalra, Monika Jain, Drishti Tiwari, Nancy Ahuja, Sumit Kumar

Project Assistant:

Puneet Kumar, Anuja Chacko

Technical Assistant:

Asheesh Kumar Khare


Past group members:



Sandeep Dave (Ph.D.), Hedwin Kitdorlang Dkhar (Ph.D.),
Sahil Mahajan (Ph.D.), Vemika Chandra (Ph.D.), Ankita Saini (Ph.D.)

 

Sambhavi, Naval JitKaur,

Arun Kumar Somavarapu,

Kanupriya Sethi.

  



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