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Beena Krishnan
Protein Design & Engineering, Protein Spectroscopy

Contact Address:

Senior Scientist (Scientist EI, Ad hoc) / Ramalingaswami Fellow
Lab No.:  P207, GNRPC
Institute of Microbial Technology,
Sector 39-A, Chandigarh – 160 036, India
Phone: +91-172-6665468 (office)
+91-172-6665482 (Lab)

Write-up of research and development interests/focus, past and present goals:


• Protein folding/mis-folding and degradation 
• Developing strategies to quantify cellular environment & to monitor processes such as protein folding in live cells
• Investigation of protein secretion systems and their role in pathogenesis
• Membrane biogenesis
• Mechanism of hijacking of host cells by RNA viruses

Current Research 

Proteins are the workhorse biomolecules of a cell. My research interest is in addressing two important aspects of proteins: protein folding mechanism at a fundamental level, and a molecular-level understanding of specific class of proteins, such as extracellular proteins, that are implicated in conformational diseases.
Immediate research themes that will be the focus of my group are:
•  Folding, mis-folding & polymerization of serpins  
•  Rational re-designing of human plasma Serpins to develop improved therapeutic biologics 
•  Designing & biophysical characterization of fluorescent protein-based biosensors
•  Understanding sequence determinants of protein folding and mis-folding

Significant recognition: Awards, fellowships, international funding of distinction, technologies transferred/licensed etc.:

Ramalingaswami Re-entry Fellowship from DBT, Govt. of India, 2012-2017
Postdoctoral Research Fellowship Grant from the Alpha-1 Foundation, 2007 – 2009, to carry out research at UMass, Amherst with Prof. Lila M. Gierasch as a mentor
Invited speaker at the 6th International Symposium on the Chemistry and Biology of Serpins at North Carolina, Chapel Hill, Sept. 2011
Invited speaker at the Gordon Research Conference on Protein Folding Dynamics, Ventura, CA, Jan. 2006
Protein Science Young Investigator Travel Grant / The Protein Society Finn Wold Travel Award
CSIR-UGC NET fellowship, Dec. 1999
Reviewer for: Chemistry – A European Journal; Chemistry – An Asian Journal; Chemical Biology and Drug Design; Process Biochemistry; PLoS ONE; & Biochemistry

Selected list of Publications and Patents:

Early folding events protect aggregation-prone regions of a β-rich protein.
Budyak IL*, Krishnan B*, Marcelino-Cruz AM, Ferrolino MC, Zhuravleva A, Gierasch LM.
(2013) Structure 21(3):476-85. (* Equal Authorship)
Maki JL, Krishnan B, Gierasch LM.
Using a low denaturant model to explore the conformational features of translocation-active SecA. (2012) Biochemistry 51(7):1369-79. (Cover)

Krishnan B and Gierasch LM.
Dynamic local unfolding in the serpin alpha-1 antitrypsin provides a mechanism for loop insertion and polymerization. (2011) Nature Structural & Molecular Biology 18(2):222-6.

Krishnan B, Kulothungan SR, Patra AK, Udgaonkar JB, Varadarajan R.
SecB-mediated protein export need not occur via kinetic partitioning. (2009)
Journal of Molecular Biology 385(4):1243-56. 

Krishnan B, Gierasch LM.
Cross-strand split tetra-Cys motifs as structure sensors in a beta-sheet protein. (2008)
Chemistry & Biology 15(10):1104-15.(Cover)

Krishnan B, Szymanska A, Gierasch LM.
Site-specific fluorescent labeling of poly-histidine sequences using a metal-chelating cysteine. (2007) Chemical Biology & Drug Design 69(1):31-40.

Past group members:

Bhawna Sharma (Quantum Solutions India)
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