Molecular Biochemistry and Microbiology
Dipak Dutta, Ph.D.
Scientist E1/ Ramanujan Fellow
Institute of Microbial Technology,
Sector 39-A, Chandigarh – 160036, India
Phone: +91-172-6665307 (office)
Write-up of research and development interests/focus, past and present goals:
• Global stress response mechanism in Bacteria.
• Importance of metal homeostasis in ribosome functioning in Escherichia coli.
• Understanding the regulation of transcription and replication processes and their cross-talk.
• Outer membrane biogenesis in Escherichia coli.
With simple genetic make-up bacteria are the most intelligent and successful organisms on the earth. This simplicity is the key to their greatest fitness in the most complex environment. Under a given stress bacteria rewire its metabolic circuitry, radically, to adapt in the environment. If adaptation fails, bacteria evolve quite easily through mutations in its own genome. My laboratory is dedicated to explore and to understand the biochemical basis of survival strategy of bacteria by looking at the functioning of central dogma machineries. The cutting edge in vivo methodologies (genetic manipulation, reporter assays, in situ footprinting, confocal microscopy etc.) coupled with in vitro techniques (transcription and replication assays, enzyme kinetics, microarray, 2D proteomics etc.) in any extent is being used to unravel the survival strategy of bacteria. With this theme, my laboratory focuses on the following ongoing projects:
1. Replisome and RNA polymerase collision on the DNA track
2. Beta-clamp of replisome in DNA repair
3. Rho dependent termination and stress response
4. Unraveling manganese stress management in E. coli.
Significant recognition: Awards, fellowships, international funding of distinction, technologies transferred/licensed etc.:
• Ramanujan Fellowship from DST, Govt. of India, 2012-2017
• Postdoctoral Research Fellowship at NYU School of Medicine, New York, USA, 2008-2012
• Invited speaker at the New York Academy of Science (NYAS), 7 World Trade Center, New York, USA, 2010. NYAS member (2010-11).
• Invited speaker for Transcription meeting at the Mountain Lake, Virginia, USA, 2010
• Invited speaker at Young Investigation Meeting, MIT, Boston, USA, 2011
• Invited speaker at Young Investigation Meeting, ILS, Bhubaneswar, India, 2011
• CSIR-NET:2000, 2001; GATE:2001 (All India Rank 13)
• Satish and Tulashi Manna Medal, University of Kalyani, 2001
Selected list of Publications and Patents:
1. Kaur G, Sengupta S, Kumar V, Kumari A, Ghosh A, Parrack P, Dutta D.*(2014) A novel MntR-independent mechanism of manganese homeostasis in Escherichia coli by the ribosome-associated protein HflX. J. Bacteriol. 196, XX-XX. (Cover illustration). *correspondence
2. Kaur G, Dutta D, Thakur KG. (2014) Crystal structure of Mycobacterium tuberculosis CarD, an essential RNA polymerase binding protein, reveals a quasi-domain swapped dimeric structural architecture. Prot Struc, Func Bioinfo 82, 879-884, (online: 17 OCT 2013)
3. Dutta D, Shatalin K, Epshtein V, Gottesman ME and Nudler E. (2011) Linking RNA Polymerase Backtracking to Genomic Instability. Cell 146, 533-543.
4. Ephstein V*, Dutta D*, Wade J, Nudler E. (2010) An allosteric mechanism of Rho-dependent termination. Nature 463, 245-249. (*Equal contribution)
5. Dutta D, Bandyopadhyay K, Datta AB, Sardesai A, Parrack P. (2009) Properties of HflX, an enigmatic protein from Escherichia coli. J. Bacteriol 191, 2307-2314.
6. Jain N, Dhimole N, Khan AR, De D, Tomar SK, Sajish M, Dutta D, Parrack P, Prakash B. (2009) Escherichia coli HflX interacts with 50S ribosomal subunits in presence of nucleotides. Biochem Biophys Res Commun 379, 201-205.
7. Dutta D, Chalissery J, Sen R. (2008). Transcription termination factor Rho prefers catalytically active elongation complexes for releasing RNA. J Biol Chem 283, 20243-51.
Present group members:
Dipak Dutta, Principal Investigator
Vineet Kumar, PhD student
Amit Bhardwaj, PhD student
Anand Prakash, PhD student
Debarghya Ghosh, Project assistant
Past group members: Aruna Thakur, Research Intern Student (2012-2014)