Dr. Barnali Chaudhuri
Structural biology; crystallography; small-angle solution scattering; hybrid approach
Write-up of research and development interests/focus, past and present goals:
Our long-term goal is to understand the molecular mechanism of chromosomal origin segregation in bacteria using in vitro reconstituted systems. In particular, we are working with the origin segregation apparatus (segrosome) from human pathogen Mycobacterium tuberculosis. Segrosome appears to act as a "Burnt-Bridge Brownian motor"; however, the exact mechanism of force generation for segrosome-mediated DNA separation is not understood. In order to achieve our goal, we combine and correlate results obtained from a number of biophysical techniques, such as macromolecular crystallography, electron microscopy and small angle solution scattering.
In addition, we are interested in understanding polar capturing of various factors and divisome assembling in mycobacteria.
Significant recognition: Awards, fellowships, international funding of distinction, technologies transferred/licensed etc.:
-Invited talk, Pittsburgh Diffraction Conference, Cleveland, USA, 2011
-Served as a member of the proposal review panel, Macromolecular Crystallography,
National Synchrotron Light Source (NSLS), Brookhaven National Lab (BNL), USA
-Served as a proposal reviewer, Small Angle Solution Neutron Scattering, Oak Ridge National Lab
-Served as a member of the Academic Council, South Asian University (SAARC)
-Invited micro-symposium co-chair, Asian Crystallography Association Meeting, 2015
-Invited micro-symposium organizer and co-chair, 24th Congress and General Assembly of
the International Union of Crystallography (IUCR), Hyderabad, 2017
Selected list of Publications and Patents:
Chaudhuri BN*. Emerging applications of small angle solution scattering in structural biology. Protein Sci. 2015 24(3):267-76. [Review article]
Qian, S, Urban, V, Dean, R and Chaudhuri BN*, The higher order organization of mycobacterial partition assembly: Does the DNA wrap a protein core? PLOS ONE, 2012, 7(12): e52690.
Chaudhuri BN*, Gupta, S., Urban, V. S., Chance, M. R., D'Mello, R. Smith, R., Lyons, K. and Gee, J. A combined global and local approach to elucidate spatial organization of the mycobacterial ParB-parS partition assembly. Biochemistry, Vol 50 (11), 2011, 1799-807.
Chaudhuri BN* and Dean R, The evidence of large-scale DNA-induced compaction in the mycobacterial chromosomal ParB. Journal of Molecular Biology, 413 (5), 2011, 901-907.
Chaudhuri, BN*, Chan, S., Perry, J. and Yeates, TO. Crystal structure of the apo forms of 55 tRNA pseudouridine synthase from M. Tuberculosis: a hinge at the base of the catalytic cleft. J Biol Chem. 2004, 279: 24585 – 91.
Chaudhuri, BN, Sawaya M, Kim CY, Waldo GS, Park MS, Terwilliger TC and Yeates, TO. The Crystal structure of the first enzyme in the pantothenate biosynthetic pathway, ketopantoate hydroxymethyltransferase, from M. Tuberculosis. Structure (Cell Press). 2003, 11:753-64.
Chaudhuri BN, Lange SC, Myers RS, Chittur SV, Davisson VJ and Smith JL. Crystal Structure of Imidazole Glycerol Phosphate Synthase: A Tunnel through a (b/a)8 Barrel Joins Two Active Sites. Structure (Cell press). 2001, 10, 987-97.
Chaudhuri BN, Kleywegt GJ, Bjorkman J, Lehman-McKeeman LD, Oliver JD, Jones TA. The structures of alpha 2u-globulin and its complex with a hyaline droplet inducer. Acta Crystallogr D Biol Crystallogr. 1999, 55:753-62.
Present group members:
Komal Choukate – Junior research fellow (graduate student)
Shelley Singh – Junior research fellow (graduate student)
Lucky Singh – Junior research fellow (graduate student)
Jagrity Choudhury – Junior research fellow (graduate student)
Anu Sodhi - Junior research fellow (DBT)
Brohmomoy Basu - Junior research fellow (DBT)
Karman Virk - CSIR research Intern