Room No. 410, 4th Floor,
Cell Biology and Immunology Division
Cell biology and immunology, hybridoma technology and antigen-antibody interactions.
Intracelluar infections- finding target molecules on the surface of pathogen and infected cells using monoclonal antibodies. Infectious diseases, malaria and tuberculosis in particular, remain the leading cause of human mortality. The objectives of our ongoing efforts are two folds: i) to identify the neo-antigenic determinants on pathogen and infected cell- surface and, ii) to characterize the identified molecules biochemically and functionally, using monoclonal antibodies (MAbs) generated by employing novel immunization strategies. We have shown that the antibodies, generated by immunization with the parasite-infected homologous cell membranes, react with new antigenic determinants on pathogen and infected cell surface both in vitro and in vivo.
Antibody specificity and cross reactivity against small molecules. Studies on the cross-reactivity and specificity of antibodies are important not only serologically but central to delineate the epitopic and functional regions on the antigen. Our lab studies are focused on antibody specificities against small molecules and polysaccharide antigens.
Applied studies. Work in this direction involves the development of monoclonal antibodies based diagnostics and therapeutics.
Kumar B., Bhalla, V, Bhadoriya, RPS, Suri, CR and Varshney GC. (2016). Label-free electrochemical detection of malaria-infected red blood cells. RSC Advances, 6, 75862-75869.
Verma D, Das L, Vandana G, Dikshit KL and Varshney GC (2015). Heterogeneity among homologs of cutinase-like protein Cut5 in mycobacteria. PLOS ONE. DOI:10.1371/journal.pone.0133186.
Bhatia R, Gautam A, Gautam S, Mehta D, Kumar V, Raghava GPS and Varshney GC (2015). Assessment of SYBR green I dye-based fluorescence assay for screening antimalarial activity of cationic peptides and DNA intercalating agents. Antimicroobial Agents and Chemotherapy 59 (5), 2886-2889.
Mehta D, Anand P, Kumar V, Joshi A, Mathur D, Singh S, Tuknait A, Chaudhary K, Gautam SK, Gautam A, Varshney GC and Raghava GPS (2014). ParaPep: a web resource for experimentally validated antiparasitic peptide sequences and their structures. Database 1–7 doi: 10.1093/database/bau051.
Ahmad S, Gupta S, Kumar R, Varshney GC and Raghava GPS (2014). Herceptin resistance database for understanding mechanism of resistance in breast cancer patients. Scientific Reports, vol 4, doi:10.1038/srep04483.